Russia says one of its warships fired warning shots at a Turkish fishing vessel in the Aegean Sea to avoid a collision.
A Russian defence ministry statement said the Turkish vessel approached to 600m (1,800ft) before turning away in response to Russian small arms fire.
The Turkish military attache in Moscow has been summoned to the foreign ministry over the incident.
Relations remain tense over Turkey’s shooting down of a Russian bomber.
The warplane crashed in Syria in last month’s clash, with Turkey maintaining it crossed into its airspace. Russia disputes this.
In the latest incident, Russia’s defence ministry said its frigate Smetlivy spotted the fishing boat when it was 1,000m (3,000ft) away heading towards the Russian vessel’s starboard side.
“Despite numerous attempts by the crew of the Smetlivy, the crew of the Turkish fishing boat did not make radio contact and did not respond to visual signals by semaphore or warning flares,” the ministry said in a statement.
To prevent a collision, “small arms were fired in the direction of the Turkish vessel from a range that is not lethal.”
Russian military vessels are involved in Russia’s intervention against rebels in Syria.